ABSTRACT
Linum usitatissimum (L.), the Flaxseed (FS) and Sesamum indicum (L.), the sesame seeds (SS) are rich sourcesof lignans, secoisolariciresinol diglucoside (SDG), and Sesamin (Sm), respectively. Synergistic effects of theSDG and Sm lignan samples were investigated in the present study, a first of its kind. The high performanceliquid chromatography fingerprint identified the presence of SDG and sesamin in FS and SS. Antimicrobialactivity of SDG+Sm combination by disc diffusion in opposition to Bacillus subtilis, Escherichia coli,Klebsiella pneumoniae, Pseudomonas aeruginosa, and Staphylococcus aureus significantly arrests growthof the bacteria in comparison to independent use of SDG and Sm. Bacterial biofilm inhibition capacity ofSDG+Sm imaged by confocal laser scanning microscopy revealed the loss of microcolonies. SDG+Sm couldinhibit the 15-LOX and COX-2 enzyme at relatively lower concentrations. Furthermore, SDG+Sm quenchedfree radicals produced by Fenton’s reagent studied by DNA-protection assay indicating its robust antioxidantproperty in protecting the DNA. These results put together encourage the use of a combination of FS and SSseed lignans SDG+Sm in a wide range of applications as natural preservatives with pharmacological effects,such as anti-inflammatory agent and aid, in their promotion as nutraceuticals.